Dr Chee Kai Chan completed his BSc and MEd from the National University of Singapore. For his PhD he worked under the supervision of Prof David Jans at the John Curtin School of Medical Research at the Australian National University (1995-2000). His project was on nuclear transport, employing novel nuclear localisation signal peptides to enhance gene delivery. He went on to a A*STAR Post-doctoral Fellowship at the Johns Hopkins School of Medicine in Baltimore under the mentoring of Professor Wade Gibson working on a project using of Bacteria Artificial Chromosomes to generate mutants of Human cytomegalovirus. He returned to Singapore to be an Assistant Professor at the Nanyang Technological University at the National Institute of Education. He subsequently moved to be a lecturer at the Department of Genetics at the La Trobe University in Melbourne, Australia, in 2003 until 2014.
During his time in La Trobe University, he lectured in a number of courses including 1st year Genetics, 2nd and 3rd year Human and Molecular Genetics, 2nd and 3rd year Medical Genetics for Medical Science students and Masters in Biotechnology. He was a course coordinator for Human and Molecular Genetics for 2nd year, a Post-graduate Coordinator of the Department of Genetics, a member of the School Teaching and Learning Committee of the School of Molecular Sciences. He was also a member of the University Genetic Manipulation Supervisory Committee and an executive member of the Food For Life Research Committee in La Trobe.
He has a passion for Human and Medical Genetics Education. For his contribution to Teaching and Learning he received the Faculty of Science, Engineering and Technology Citation Award in 2007.
He was also active as a Genetics Director in an Australian based company called Fitgenes Pty Ltd from 2008-2014 where he presently remains as a consultant on the company’s Scientific Advisory Committee.
Dr Chan’s research focus is 2 fold
1) Enhancing gene therapy, gene and drug delivery for treatment of beta thalassemia and cancer
We are working to develop novel non-viral gene delivery vectors and strategies to enhance mitochondrial and nuclear delivery for gene therapy. To test for the effectiveness and usefulness of these novel gene delivery strategies we have chosen beta-thalassemia and cancer as disease models.
We have also investigated the use of various polymeric nanoparticle encapsulation strategies and the use of ultrasound to improve non-invasive and targeted delivery of therapeutic drugs, genes and artificial chromosome to treat and to correct genetic defects.
2) Nutrigenomics. The interaction between micronutrients as genetic regulators and key genes that impact various aspects of mental health and metabolic disease.
In this era in which the accessibility and cost of having our genomes sequenced is constantly getting closer to within the reach of medical research labs, we are now poised to take advantage of this to look at how genetic and epigenetic changes can lead to alterations in gene expression at the personal level and at the community level. We are now able to ask questions as to how these variations can be modulated by micronutrients and lifestyle changes and more importantly what the consequences are in health and diseases. At the translational level we are looking at the interaction of nutrition and lifestyle changes with an individual’s genetic variation in the context two aspects of health and disease
1) mental health and diseases such as Autism and Alzheimer’s
2) metabolic syndrome such as diabetes and Non-Alcoholic Fatty Liver Disease.
We are also interested in understanding the molecular mechanisms of such interactions and we are using the advantages of the zebrafish as a powerful model to provide us with valuable clues to address our hypotheses. The zebrafish has several crucial and relevant advantages, its rapid growth and development, ease in maintenance. Moreover, its close homology at the genetic, organ and tissue level to humans, its transparency at its embryonic stage allows for tractable observation at the cellular level in vivo. As such it is able to provide a model that is extremely useful to us in addressing questions regarding molecular mechanisms and in the understanding, the treatment and the progress of health and diseases.
Beta-globin switching during embryonic stem cell differentiation
In collaboration with La Trobe's Dr Adam Hart and Dr Jim Vadolas at the Murdoch Children’s Research Institute in Melbourne Australia, we are investigating the various molecular mechanisms that regulate the embryonic switch from gamma-globin gene expression to beta-globin with the objective of finding ways to compensate for the loss of beta-globin in adult thalassemia patients.
Sports and nutrigenomics. Together with Dr Regina Belski, Dr Michael Kingsley of La Trobe University, and other collaborators we are looking at how caffeine supplements impact performance and recovery of team sports such as football as well as individual sports such as cycling.
Obesity and metabolic diseases. A continuing collaboration examining various aspects of nutrition impacting obesity, diabetes, non-alcoholic fatty liver disease involves Drs Catherine Itsiopoulos, Audrey Tierney, Mark Jois from the Food for Life, Health and Performance program in La Trobe University.
Alzheimer’s and nutrigenomics-We have an initiated a trial looking at nutrition, epigenetics and the delay in the progress of dementia and Alzheimer’s using bioactive nutrients with Prof Ralph Martins and Kathryn Goozee in Sydney.
1. Stephen R Doyle, Ph.D.; Naga R.P. Kasinadhuni; Chee-Kai Chan; Warwick N Grant (2013) Evidence of evolutionary constraints that influences the sequence composition and diversity of mitochondrial matrix targeting signals. PLos ONE 8(6). e67938.doi:10.1371/journal.pone.0067938.
2. Duy Huynh; Dilara Akcora; Jordane Malaterre; Chee-Kai Chan; Xu-Ming Dai; Ivan Bertoncello; E. Richard Stanley; Robert George Ramsay (2013) CSF1 Receptor-Dependent colon development, homeostasis and inflammatory stress response. PLoS ONE 8 (2) e56951.doi:10.1371/journal.pone.0056951
3. Stephen R Doyle, Chee Kai Chan and Warwick Grant (2011) Enhanced annealing of mismatched oligonucleotides using a novel melting curve assay allows efficient in vitro discrimination and restriction of a single nucleotide polymorphism. BMC Biotechnology, 11:83doi:10.1186/1472-6750-11-83
4. Michal Ugarenko, Chee-Kai Chan, Abraham Nudelman, Ada Rephaeli, Suzanne M Cutts, Don R Phillips (2009). Development of pluronic micelle-encapsulated doxorubicin and formaldehyde-releasing prodrugs for localized anticancer chemotherapy. Oncology Research. 17(7): 283-99
5. Duy Huynh, Xu-Ming Dai, Sayan Nandi, Sally Lightowler, Melanie Trivett, Chee-Kai Chan, Ivan Bertoncello, Robert G Ramsay, E Richard Stanley. (2009) Colony stimulating factor-1 dependence of paneth cell development in the mouse small intestine. Gastroenterology. 137(1): 136-44, 144.
6. Stephen R Doyle, Chee Kai Chan (2008). Mitochondrial gene therapy: an evaluation of strategies for the treatment of mitochondrial DNA disorders. Human Gene Therapy. Dec;19(12): 1335-48
7. Stephen R Doyle, Chee Kai Chan (2007). Differential intracellular distribution of DNA complexed with polyethylenimine (PEI) and PEI-polyarginine PTD influences exogenous gene expression within live COS-7 cells. Genetic Vaccines and Therapy. 5: 11
8. Duy D. Huynh, E. Vincan, T. Mantamadiotis, D. Purcell, CK Chan R. Ramsay (2007) Oncogenic properties of HIV-Tat in colorectal cancer cells. Current HIV Research, 5(4): 403-9.
9. Tania Blanksby & Chee Kai Chan (2006) Feedback assessment of Science report writing for first year Genetics students . UNISERVE Symposium, Assessment in Science Teaching and Learning. 161.
10. Loveland, A.N. Chee-Kai Chan, Edward J. Brignole and Wade Gibson (2005) Cleavage of human cytomegalovirus protease pUL80a at internal and cryptic sites is not essential but enhances infectivity. J Virol 79 (20), 12961-12968
11. Duy K. Pham and Chee Kai Chan. (2005) Impact of Polymeric Surfaces on Oligonucleotides Immobilization for Lab-On-A-Chip Applications, Trends in DNA Research (Nova Science Publishers) Edit: Corey Woods
12. Chan, C.K. et al. (2002) Cytomegalovirus assemblin (pUL80a): cleavage at internal site not essential for virus growth; proteinase absent from virions. J Virol 76 (17), 8667-8674
13. Chan, C.K. and Jans, D.A. (2002) Using nuclear targeting signals to enhance non-viral gene transfer. Immunol Cell Biol 80 (2), 119-130
14. Chan, C.K. and Jans, D.A. (2001) Enhancement of MSH receptor- and GAL4-mediated gene transfer by switching the nuclear import pathway. Gene Ther 8 (2), 166-171
15. Chan, C.K. et al. (2000) Supramolecular structure and nuclear targeting efficiency determine the enhancement of transfection by modified polylysines. Gene Ther 7 (19), 1690-1697
16. Hubner, S. et al. (1999) Plant importin alpha binds nuclear localization sequences with high affinity and can mediate nuclear import independent of importin beta. J Biol Chem 274 (32), 22610-22617
17. Chan, C.K. and Jans, D.A. (1999) Enhancement of polylysine-mediated transferrinfection by nuclear localization sequences: polylysine does not function as a nuclear localization sequence. Hum Gene Ther 10 (10), 1695-1702
18. Chan, C.K. and Jans, D.A. (1999) Synergy of importin alpha recognition and DNA binding by the yeast transcriptional activator GAL4. FEBS Lett 462 (1-2), 221-224
19. Jans, D.A. et al. (1998) Signals mediating nuclear targeting and their regulation: application in drug delivery. Med Res Rev 18 (4), 189-223
20. Chan, C.K. et al. (1998) Mutual exclusivity of DNA binding and nuclear localization signal recognition by the yeast transcription factor GAL4: implications for nonviral DNA delivery. Gene Ther 5 (9), 1204-1212
Human and Medical Genetics Education
1.Chee Kai Chan, Adam Hart, Anna Lister, Stephen Doyle (2011). Learning styles of 3rd year Genetics Students and their Affection and Perception of effectiveness of a range of Instructional Modes. Proceedings of the Australian Conference on Science and mathematics Education. 121.
2. Tania Blanksby & Chee Kai Chan (2006) Feedback assessment of Science report writing for first year Genetics students. UNISERVE Symposium, Assessment in Science Teaching and Learning. 161.
3. Goh NK, Chan CK & Chia LS. (1995). A comparison of the differences of two teaching strategies in the modification of students misconceptions in science. ACCESS, 13(2); 161-171.
4. Goh NK, Chan CK & Chia LS. (1995). Gender differences, misconceptions and instruction in Science. Singapore J. Edu. 15: 33-41.